Fig. 2. Modulation of mitochondrial metabolism by Src according to nutrient availability. (a, b) Levels of blood glucose (a) and liver glycogen (b) in fed and fasted Src^+/+ and Src^-/- mice (n=3). (c-e) Oxygen consumption rates of liver mitochondria obtained from fed and fasted Src^+/+ and Src^-/- mice (n=7) in presence of specific substrates and inhibitors to evaluate complex CI+III+IV-dependent respiration (c), CI+II+III+IV-dependent respiration (d), and CIV-dependent respiration (e). (f-i) Enzymatic activities of complexes I+III (f), complex II (g), complex IV (h) and citrate synthase (i) in liver of fed and fasted Src^+/+ and Src^-/- mice (n=4). (j) Representative immunoblots (n=4) of Src, SDHa, ATP5a, NDUFA9, SOD2, TOM20 and Cytc in liver of fed and fasted Src^+/+ and Src^-/- mice. See Fig. S1c for quantification. (k) H₂O₂ production by liver mitochondria isolated from fed and fasted Src^+/+ and Src^-/- mice (n=5). Data are presented as mean ± s.e.m. *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001 determined by a two-way ANOVA followed by post-hoc Tukey's test.